Unit

UNIT 9.1 Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries

  1. Barry Vant-Hull,
  2. Larry Gold,
  3. Dominic A. Zichi

Published Online: 1 FEB 2000

DOI: 10.1002/0471142700.nc0901s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Vant-Hull, B., Gold, L. and Zichi, D. A. 2000. Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries. Current Protocols in Nucleic Acid Chemistry. 00:9.1:9.1.1–9.1.16.

Author Information

  1. NeXstar Pharmaceuticals, Boulder, Colorado

Publication History

  1. Published Online: 1 FEB 2000
  2. Published Print: MAY 2001

Abstract

A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic evolution of ligands by exponential enrichment (SELEX). This overview unit discusses nucleic acid libraries that can be used, affinity probability distributions, an equilibrium model for SELEX, and optimal conditions including concentrations and signal-to-noise ratios.