UNIT 9.2 Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
Published Online: 1 DEC 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Hall, B., Micheletti, J. M., Satya, P., Ogle, K., Pollard, J. and Ellington, A. D. 2009. Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection. Current Protocols in Nucleic Acid Chemistry. 39:9.2:9.2.1–9.2.28.
- Published Online: 1 DEC 2009
- Published Print: DEC 2009
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions. Curr. Protoc. Nucleic Acid Chem. 39:9.2.1-9.2.28. © 2009 by John Wiley & Sons, Inc.
- In vitro selection;
- DNA pool synthesis;
- phosphoramidite DNA synthesis;