Unit

UNIT 9.4 Selection for Catalytic Function with Nucleic Acids

  1. Ronald R. Breaker

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0904s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Breaker, R. R. 2001. Selection for Catalytic Function with Nucleic Acids. Current Protocols in Nucleic Acid Chemistry. 00:9.4:9.4.1–9.4.17.

Author Information

  1. Yale University, New Haven, Connecticut

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in vitro selection experiments for catalytic function. It outlines several representative protocols as examples of successful selection experiments, providing a conceptual basis for the design and implementation of new selective-amplification protocols for nucleic acids.