Unit

UNIT 9.5 In Vitro Selection of RNA Aptamers to a Small Molecule Target

  1. Vlad Codrea1,
  2. Michelle Hayner2,
  3. Bradley Hall2,
  4. Sulay Jhaveri3,
  5. Andrew Ellington1

Published Online: 1 MAR 2010

DOI: 10.1002/0471142700.nc0905s40

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Codrea, V., Hayner, M., Hall, B., Jhaveri, S. and Ellington, A. 2010. In Vitro Selection of RNA Aptamers to a Small Molecule Target. Current Protocols in Nucleic Acid Chemistry. 40:9.5:9.5.1–9.5.23.

Author Information

  1. 1

    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas

  2. 2

    Freshman Research Initiative, University of Texas, Austin, Texas

  3. 3

    Finnegan, Washington, D.C.

Publication History

  1. Published Online: 1 MAR 2010
  2. Published Print: MAR 2010

Abstract

This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection. Curr. Protoc. Nucleic Acid Chem. 40:9.5.1-9.5.23. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • aptamer;
  • affinity matrix;
  • target immobilization;
  • small molecule