UNIT 9.5 In Vitro Selection of RNA Aptamers to a Small Molecule Target
Published Online: 1 MAR 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Codrea, V., Hayner, M., Hall, B., Jhaveri, S. and Ellington, A. 2010. In Vitro Selection of RNA Aptamers to a Small Molecule Target. Current Protocols in Nucleic Acid Chemistry. 40:9.5:9.5.1–9.5.23.
- Published Online: 1 MAR 2010
- Published Print: MAR 2010
This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection. Curr. Protoc. Nucleic Acid Chem. 40:9.5.1-9.5.23. © 2010 by John Wiley & Sons, Inc.
- affinity matrix;
- target immobilization;
- small molecule