Unit

UNIT 9.6 In Vitro Selection Using Modified or Unnatural Nucleotides

  1. Scott M. Knudsen,
  2. Michael P. Robertson,
  3. Andrew D. Ellington

Published Online: 1 FEB 2002

DOI: 10.1002/0471142700.nc0906s07

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Knudsen, S. M., Robertson, M. P. and Ellington, A. D. 2002. In Vitro Selection Using Modified or Unnatural Nucleotides. Current Protocols in Nucleic Acid Chemistry. 7:9.6:9.6.1–9.6.21.

Author Information

  1. University of Texas, Austin, Texas

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

This is not the most recent version of the article. View current version (26 MAR 2014)

Abstract

The use of modified nucleotides in an RNA or DNA pool to be used for in vitro selection offers many potential advantages, such as the increased stability of the selected nucleic acid against nuclease degradation. This unit provides useful information and protocols for in vitro selection using modified nucleotides. It includes a discussion of when to use modified nucleotides; protocols for preparing a modified RNA pool and verifying its suitability for in vitro selection; and protocols for selecting and amplifying a functionally enriched pool.