UNIT 9.9 Compartmentalized Self-Tagging for In Vitro-Directed Evolution of XNA Polymerases
Published Online: 24 JUN 2014
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Pinheiro, V. B., Arangundy-Franklin, S. and Holliger, P. 2014. Compartmentalized Self-Tagging for In Vitro-Directed Evolution of XNA Polymerases. Current Protocols in Nucleic Acid Chemistry. 57:9.9:9.9.1–9.9.18.
- Published Online: 24 JUN 2014
Template-dependent synthesis of xenobiotic nucleic acids (XNAs) is an essential step for the development of functional XNA molecules, as it enables Darwinian evolution to be carried out with novel genetic polymers. The extraordinary substrate specificity of natural DNA polymerases greatly restricts the spectrum of XNAs available, thus making it necessary to identify DNA polymerase variants capable of incorporating a wider range of substrates. This unit summarizes compartmentalized self-tagging (CST), a directed evolution strategy developed for the selection of DNA polymerase variants capable of XNA synthesis. Curr. Protoc. Nucleic Acid Chem. 57:9.9.1-9.9.18. © 2014 by John Wiley & Sons, Inc.
- DNA polymerases;
- in vitro-directed evolution;
- synthetic nucleic acids;
- compartmentalized self-tagging;