Unit

UNIT 9.9 Compartmentalized Self-Tagging for In Vitro-Directed Evolution of XNA Polymerases

  1. Vitor B. Pinheiro1,2,
  2. Sebastian Arangundy-Franklin3,
  3. Philipp Holliger3

Published Online: 24 JUN 2014

DOI: 10.1002/0471142700.nc0909s57

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pinheiro, V. B., Arangundy-Franklin, S. and Holliger, P. 2014. Compartmentalized Self-Tagging for In Vitro-Directed Evolution of XNA Polymerases. Current Protocols in Nucleic Acid Chemistry. 57:9.9:9.9.1–9.9.18.

Author Information

  1. 1

    Institute of Structural and Molecular Biology, University College, University of London, London, United Kingdom

  2. 2

    Birkbeck College, University of London, London, United Kingdom

  3. 3

    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

Publication History

  1. Published Online: 24 JUN 2014

Abstract

Template-dependent synthesis of xenobiotic nucleic acids (XNAs) is an essential step for the development of functional XNA molecules, as it enables Darwinian evolution to be carried out with novel genetic polymers. The extraordinary substrate specificity of natural DNA polymerases greatly restricts the spectrum of XNAs available, thus making it necessary to identify DNA polymerase variants capable of incorporating a wider range of substrates. This unit summarizes compartmentalized self-tagging (CST), a directed evolution strategy developed for the selection of DNA polymerase variants capable of XNA synthesis. Curr. Protoc. Nucleic Acid Chem. 57:9.9.1-9.9.18. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • DNA polymerases;
  • in vitro-directed evolution;
  • synthetic nucleic acids;
  • XNA;
  • compartmentalized self-tagging;
  • CST