UNIT 10.5 Analysis and Purification of Synthetic Nucleic Acids Using HPLC
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Andrus, A. and Kuimelis, R. G. 2001. Analysis and Purification of Synthetic Nucleic Acids Using HPLC. Current Protocols in Nucleic Acid Chemistry. 1:10.5:10.5.1–10.5.13.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
This is not the most recent version of the article. View current version (3 JUN 2015)
HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed-phase HPLC allows a high-capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion-exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed-phase and anion-exchange HPLC, it can be used for oligonucleotides of up to ˜50 nt in length.