Unit

UNIT 10.5 Analysis and Purification of Synthetic Nucleic Acids Using HPLC

  1. Alex Andrus1,
  2. Robert G. Kuimelis2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc1005s01

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Andrus, A. and Kuimelis, R. G. 2001. Analysis and Purification of Synthetic Nucleic Acids Using HPLC. Current Protocols in Nucleic Acid Chemistry. 1:10.5:10.5.1–10.5.13.

Author Information

  1. 1

    PE Applied Biosystems, Foster City, California

  2. 2

    Phylos, Inc., Lexington, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

This is not the most recent version of the article. View current version (3 JUN 2015)

Abstract

HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed-phase HPLC allows a high-capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion-exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed-phase and anion-exchange HPLC, it can be used for oligonucleotides of up to ˜50 nt in length.