UNIT 10.7 Cartridge Methods for Oligonucleotide Purification
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Andrus, A. and Kuimelis, R. G. 2001. Cartridge Methods for Oligonucleotide Purification. Current Protocols in Nucleic Acid Chemistry. 1:10.7:10.7.1–10.7.5.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
Protocols are given for purification of oligonucleotides by dimethoxytrityl-sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid oligonucleotide purification, clean-up by selective adsorption, and elution on solid-phase media. Many of these products are prepackaged, single-use cartridges or columns filled with affinity or size-exclusion media.