Unit

UNIT 10.7 Cartridge Methods for Oligonucleotide Purification

  1. Alex Andrus1,
  2. Robert G. Kuimelis2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc1007s01

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Andrus, A. and Kuimelis, R. G. 2001. Cartridge Methods for Oligonucleotide Purification. Current Protocols in Nucleic Acid Chemistry. 1:10.7:10.7.1–10.7.5.

Author Information

  1. 1

    PE Applied Biosystems, Foster, California

  2. 2

    Phylos, Inc., Lexington, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

Protocols are given for purification of oligonucleotides by dimethoxytrityl-sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid oligonucleotide purification, clean-up by selective adsorption, and elution on solid-phase media. Many of these products are prepackaged, single-use cartridges or columns filled with affinity or size-exclusion media.