UNIT 11.3 Thermal Methods for the Analysis of RNA Folding Pathways
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Draper, D. E., Bukhman, Y. V. and Gluick, T. C. 2001. Thermal Methods for the Analysis of RNA Folding Pathways. Current Protocols in Nucleic Acid Chemistry. 2:11.3.1–11.3.13.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
Once a model of the secondary structure of an RNA has been deduced, thermal melting analysis can be used to determine whether the model accounts for all intramolecular interactions of the RNA, or whether noncanonical and tertiary interactions make the structure more stable than predicted, or link parts of the structure in unexpected ways. It is also useful to determine the pH, salt, and temperature ranges under which the RNA adopts a stably folded structure, or to analyze unfolding pathways. This unit discusses sample preparation, instrumentation, and theoretical background. It also provide a sample analysis of tRNA unfolding.