Unit

UNIT 11.3 Thermal Methods for the Analysis of RNA Folding Pathways

  1. David E. Draper,
  2. Yury V. Bukhman,
  3. Thomas C. Gluick

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc1103s02

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Draper, D. E., Bukhman, Y. V. and Gluick, T. C. 2001. Thermal Methods for the Analysis of RNA Folding Pathways. Current Protocols in Nucleic Acid Chemistry. 2:11.3:11.3.1–11.3.13.

Author Information

  1. Johns Hopkins University, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Once a model of the secondary structure of an RNA has been deduced, thermal melting analysis can be used to determine whether the model accounts for all intramolecular interactions of the RNA, or whether noncanonical and tertiary interactions make the structure more stable than predicted, or link parts of the structure in unexpected ways. It is also useful to determine the pH, salt, and temperature ranges under which the RNA adopts a stably folded structure, or to analyze unfolding pathways. This unit discusses sample preparation, instrumentation, and theoretical background. It also provide a sample analysis of tRNA unfolding.