Unit

UNIT 11.6 Time-Resolved Hydroxyl Radical Footprinting of RNA with X-Rays

  1. Sarah A. Woodson1,
  2. Michael L. Deras1,
  3. Michael Brenowitz2

Published Online: 1 NOV 2001

DOI: 10.1002/0471142700.nc1106s06

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Woodson, S. A., Deras, M. L. and Brenowitz, M. 2001. Time-Resolved Hydroxyl Radical Footprinting of RNA with X-Rays. Current Protocols in Nucleic Acid Chemistry. 6:11.6:11.6.1–11.6.24.

Author Information

  1. 1

    Johns Hopkins University, Baltimore, Maryland

  2. 2

    Albert Einstein College of Medicine of Yeshiva University, Bronx, New York

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: SEP 2001

Abstract

RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X-ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.