UNIT 11.9 Use of Chemical Modification To Elucidate RNA Folding Pathways
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Mathews, D. H. and Turner, D. H. 2002. Use of Chemical Modification To Elucidate RNA Folding Pathways. Current Protocols in Nucleic Acid Chemistry. 9:11.9:11.9.1–11.9.4.
- Published Online: 1 AUG 2002
- Published Print: JUN 2002
As discussed in this overview, chemical modification is sensitive to the accessibility of a nucleotide to the solvent, and many nucleotides become less accessible as an RNA folds into its structured form. Chemical modification reagents are therefore suitable for following RNA folding, and can be used to study the kinetics of structure formation on time scales ranging from minutes to hours.