UNIT 11.12 RNA Intramolecular Dynamics by Single-Molecule FRET
Published Online: 1 SEP 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Hengesbach, M., Kobitski, A., Voigts-Hoffmann, F., Frauer, C., Nienhaus, G. U. and Helm, M. 2008. RNA Intramolecular Dynamics by Single-Molecule FRET. Current Protocols in Nucleic Acid Chemistry. 34:11.12:11.12.1–11.12.22.
- Published Online: 1 SEP 2008
- Published Print: SEP 2008
Investigation of single RNA molecules using fluorescence resonance energy transfer (FRET) is a powerful approach to investigate dynamic and thermodynamic aspects of the folding process of a given RNA. Its application requires interdisciplinary work from the fields of chemistry, biochemistry, and physics. The present work gives detailed instructions on the synthesis of RNA molecules labeled with two fluorescent dyes interacting by FRET, as well as on their investigation by single-molecule fluorescence spectroscopy. Curr. Protoc. Nucleic Acid Chem. 34:11.12.1-11.12.22. © 2008 by John Wiley & Sons, Inc.
- fluorescence resonance energy transfer (FRET);
- single molecule;
- splint ligation;
- fluorescent dyes;
- nucleotide modifications;
- RNA folding