Unit

UNIT 11.13 Characterization of Conformational Dynamics of Bistable RNA by Equilibrium and Non-Equilibrium NMR

  1. Boris Fürtig1,
  2. Anke Reining1,
  3. Florian Sochor1,
  4. Eva Marie Oberhauser2,
  5. Alexander Heckel2,
  6. Harald Schwalbe1

Published Online: 19 DEC 2013

DOI: 10.1002/0471142700.nc1113s55

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Fürtig, B., Reining, A., Sochor, F., Oberhauser, E. M., Heckel, A. and Schwalbe, H. 2013. Characterization of Conformational Dynamics of Bistable RNA by Equilibrium and Non-Equilibrium NMR. Current Protocols in Nucleic Acid Chemistry. 11:11.13:11.13.1–11.13.16.

Author Information

  1. 1

    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany

  2. 2

    Institute of Organic Chemistry and Chemical Biology, Cluster of Excellence Macromolecular Complexes, Johann Wolfgang Goethe University, Frankfurt, Germany

Publication History

  1. Published Online: 19 DEC 2013

ABSTRACT

Unlike proteins, a given RNA sequence can adopt more than a single conformation. The two (or more) conformations are long-lived and have similar stabilities, but interconvert only slowly. Such bi- or multistability is often linked to the biological functions of the RNA. This unit describes how nuclear magnetic resonance (NMR) spectroscopy can be used to characterize the conformational dynamics of bistable RNAs. Curr. Protoc. Nucleic Acid Chem. 55:11.13.1-11.13.16. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • bistable RNA;
  • RNA folding;
  • real-time NMR;
  • exchange spectroscopy;
  • photo-caged RNA