UNIT 12.1 Key Experimental Approaches in DNA Nanotechnology

  1. Nadrian C. Seeman

Published Online: 1 AUG 2002

DOI: 10.1002/0471142700.nc1201s09

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Seeman, N. C. 2002. Key Experimental Approaches in DNA Nanotechnology. Current Protocols in Nucleic Acid Chemistry. 9:12.1:12.1.1–12.1.14.

Author Information

  1. New York University, New York, New York

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUN 2002


DNA nanotechnology combines unusual DNA motifs with sticky-ended cohesion to build polyhedral objects, topological targets, nanomechanical devices, and both crystalline and aperiodic arrays. The goal of DNA nanotechnology is control of the structure of macroscopic matter on the finest possible scale. Applications are expected to arise in the areas of X-ray crystallography, nanoelectronics, nanorobotics, and DNA-based computation. DNA and its close molecular relatives appear extremely well suited for these goals. This overview covers the generation of new DNA motifs, construction methods (synthesis, hybridization, phosphorylation, ligation), and a variety of methods for characterization of motifs, devices, and arrays. Finally, the use of DNA nanotechnology as a tool in biochemistry is discussed.