Unit

UNIT 12.3 Synthesis of 5′-O-Phosphoramidites with a Photolabile 3′-O-Protecting Group

  1. Markus Beier,
  2. Jörg D. Hoheisel

Published Online: 1 SEP 2004

DOI: 10.1002/0471142700.nc1203s17

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Beier, M. and Hoheisel, J. D. 2004. Synthesis of 5′-O-Phosphoramidites with a Photolabile 3′-O-Protecting Group. Current Protocols in Nucleic Acid Chemistry. 17:12.3:12.3.1–12.3.10.

Author Information

  1. Deutsches Krebsforschungszentrum, Heidelberg, Germany

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: JUN 2004

Abstract

This unit describes the chemical synthesis of phosphoramidite building blocks that carry a protecting group at the 3′ position. These inversely oriented synthons expose a 2-(2-nitrophenyl)propoxycarbonyl (NPPOC) group as the photolabile protecting group of choice. Among other applications, the building blocks can be employed for light-controlled in situ synthesis of DNA microarrays, producing arrayed oligonucleotides that are attached to the support via their 5′ ends, leaving their 3′ termini available to act as substrates for polymerases.

Keywords:

  • photolabile protecting group;
  • phosphoramidite;
  • DNA microarray