Unit

UNIT 12.4 Derivatization of Glass and Polypropylene Surfaces

  1. Markus Beier1,
  2. Jörg D. Hoheisel2

Published Online: 1 OCT 2004

DOI: 10.1002/0471142700.nc1204s18

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Beier, M. and Hoheisel, J. D. 2004. Derivatization of Glass and Polypropylene Surfaces. Current Protocols in Nucleic Acid Chemistry. 18:12.4:12.4.1–12.4.8.

Author Information

  1. 1

    Febit AG, Mannheim, Germany

  2. 2

    Deutsches Krebsforschungszentrum, Heidelberg, Germany

Publication History

  1. Published Online: 1 OCT 2004
  2. Published Print: SEP 2004

Abstract

This unit describes the derivatization of solid support media, particularly glass and polypropylene, with a linker system by an iterative process that forms a dendrimeric structure, thereby increasing stepwise the loading capacity of the surface. The procedure permits the production of various types of linkers whose characteristics can be tailored to the requirements of the eventual applications. The specific modifications described here are used either for attaching prefabricated DNA oligonucleotides, PCR products, and peptide nucleic acid (PNA) oligomers, or for in situ synthesis of DNA microarrays.

Keywords:

  • solid support;
  • etching;
  • silanization;
  • linker;
  • immobilization of biomolecules;
  • microarray