Unit

UNIT 12.5 DNA Microarray Preparation by Light-Controlled In Situ Synthesis

  1. Markus Beier,
  2. Jörg D. Hoheisel

Published Online: 1 APR 2005

DOI: 10.1002/0471142700.nc1205s20

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Beier, M. and Hoheisel, J. D. 2005. DNA Microarray Preparation by Light-Controlled In Situ Synthesis. Current Protocols in Nucleic Acid Chemistry. 20:12.5:12.5.1–12.5.10.

Author Information

  1. Deutsches Krebsforschungszentrum, Heidelberg, Germany

Publication History

  1. Published Online: 1 APR 2005
  2. Published Print: MAR 2005

Abstract

This unit describes the in situ synthesis of DNA microarrays using a light-controlled process. In a highly parallel fashion, multiple oligonucleotide probes are created from scratch on a glass support using phosphoramidite building blocks carrying a photolabile protecting group. Spatial resolution is achieved by carefully controlling the illumination of defined spots on the glass substrate, on which the oligonucleotide probes are synthesized using straightforward phosphoramidite chemistry.

Keywords:

  • oligonucleotide microarrays;
  • genomics;
  • biochip;
  • photolithography;
  • DNA synthesizer;
  • photolabile;
  • NPPOC