UNIT 12.5 DNA Microarray Preparation by Light-Controlled In Situ Synthesis
Published Online: 1 APR 2005
Copyright © 2005 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Beier, M. and Hoheisel, J. D. 2005. DNA Microarray Preparation by Light-Controlled In Situ Synthesis. Current Protocols in Nucleic Acid Chemistry. 20:12.5:12.5.1–12.5.10.
- Published Online: 1 APR 2005
- Published Print: MAR 2005
This unit describes the in situ synthesis of DNA microarrays using a light-controlled process. In a highly parallel fashion, multiple oligonucleotide probes are created from scratch on a glass support using phosphoramidite building blocks carrying a photolabile protecting group. Spatial resolution is achieved by carefully controlling the illumination of defined spots on the glass substrate, on which the oligonucleotide probes are synthesized using straightforward phosphoramidite chemistry.
- oligonucleotide microarrays;
- DNA synthesizer;