UNIT 12.9 DNA Origami: Synthesis and Self-Assembly

  1. Arivazhagan Rajendran1,2,
  2. Masayuki Endo2,3,
  3. Hiroshi Sugiyama1,2,3

Published Online: 1 MAR 2012

DOI: 10.1002/0471142700.nc1209s48

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Rajendran, A., Endo, M. and Sugiyama, H. 2012. DNA Origami: Synthesis and Self-Assembly. Current Protocols in Nucleic Acid Chemistry. 48:12.9:12.9.1–12.9.18.

Author Information

  1. 1

    Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, Japan

  2. 2

    CREST, Japan Science and Technology Corporation (JST), Tokyo, Japan

  3. 3

    Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan

Publication History

  1. Published Online: 1 MAR 2012
  2. Published Print: MAR 2012


DNA origami is an emerging technology for designing defined two- and three-dimensional (2D and 3D) DNA nanostructures. Here, we report an introductory practical guide with step-by-step experimental details for the design and synthesis of origami structures, and their size expansion in 1D and 2D space by means of self-assembly. Curr. Protoc. Nucleic Acid Chem. 48:12.9.1-12.9.18. © 2012 by John Wiley & Sons, Inc.


  • DNA origami;
  • designed nanospace;
  • self-assembly;
  • DNA nanotechnology;
  • atomic force microscopy