UNIT 13.1 Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Johnson, D. C. and Widlanski, T. S. 2004. Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates. Current Protocols in Nucleic Acid Chemistry. 15:13.1:13.1.1–13.1.31.
- Published Online: 1 FEB 2004
- Published Print: DEC 2003
This overview summarizes methodology used for the synthesis of nucleoside mono-, di-, and triphosphates. Selected techniques such as the Mitsunobu reaction, displacement reactions involving nucleoside 5′-tosylates, “anion-exchange” techniques, and phosphoramidite and phosphoramidate methodologies are highlighted. The chemistry of phosphorylation is detailed with respect to advantages and limitations under various conditions. Applicability of the methods toward the synthesis of analogs such as imidophosphates, phosphorothioates, and radiolabeled nucleotides is also addressed.
- nucleoside monophosphate;
- nucleoside diphosphate;
- nucleoside triphosphate;
- nucleotide synthesis;
- nucleotide review