Unit

UNIT 13.1 Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates

  1. David C. Johnson II,
  2. Theodore S. Widlanski

Published Online: 1 FEB 2004

DOI: 10.1002/0471142700.nc1301s15

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Johnson, D. C. and Widlanski, T. S. 2004. Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates. Current Protocols in Nucleic Acid Chemistry. 15:13.1:13.1.1–13.1.31.

Author Information

  1. Indiana University, Bloomington, Indiana

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: DEC 2003

Abstract

This overview summarizes methodology used for the synthesis of nucleoside mono-, di-, and triphosphates. Selected techniques such as the Mitsunobu reaction, displacement reactions involving nucleoside 5′-tosylates, “anion-exchange” techniques, and phosphoramidite and phosphoramidate methodologies are highlighted. The chemistry of phosphorylation is detailed with respect to advantages and limitations under various conditions. Applicability of the methods toward the synthesis of analogs such as imidophosphates, phosphorothioates, and radiolabeled nucleotides is also addressed.

Keywords:

  • nucleoside monophosphate;
  • nucleoside diphosphate;
  • nucleoside triphosphate;
  • nucleotide synthesis;
  • phosphorothioate;
  • imidophosphate;
  • nucleotide review