UNIT 13.4 Nucleoside-5′-Phosphoimidazolides: Reagents for Facile Synthesis of Dinucleoside Pyrophosphates
Published Online: 1 JAN 2006
Copyright © 2005 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Chen, L., Rejman, D., Bonnac, L., Pankiewicz, K. W. and Patterson, S. E. 2006. Nucleoside-5′-Phosphoimidazolides: Reagents for Facile Synthesis of Dinucleoside Pyrophosphates. Current Protocols in Nucleic Acid Chemistry. 23:13.4:13.4.1–13.4.10.
- Published Online: 1 JAN 2006
- Published Print: DEC 2005
A facile method is presented for preparation of dinucleoside pyrophosphate derivatives based on reaction of a nucleoside 5′-monophosphate with carbonyldiimidazole followed by treatment of the resulting nucleoside 5′-phosphoimidazolide with a nucleoside 5′-phosphate. This method is suitable for preparation of pyrophosphates analogous to NAD, FAD, and related natural pyrophosphates. The resulting compounds are useful for mechanistic studies of enzymes that use natural pyrophosphates as co-factors or substrates, and in development of inhibitors that have potential applications as therapeutic agents.
- NAD analog;
- nucleoside pyrophosphate;