UNIT 13.11 Rapid and Efficient Synthesis of Nucleoside Polyphosphates and Their Conjugates Using Sulfonyl Imidazolium Salts
Published Online: 1 DEC 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Mohamady, S. and Taylor, S. D. 2012. Rapid and Efficient Synthesis of Nucleoside Polyphosphates and Their Conjugates Using Sulfonyl Imidazolium Salts. Current Protocols in Nucleic Acid Chemistry. 51:13.11:13.11.1–13.11.24.
- Published Online: 1 DEC 2012
This unit describes a method for preparing nucleoside polyphosphates and their conjugates such as nucleoside triphosphates, symmetrical and unsymmetrical dinucleoside polyphosphates and sugar nucleotides. The protocols employ sulfonyl imidazolium salts (SnISs) as coupling reagents. These reagents are prepared in two steps in very high yield from commercially available materials and can be stored for at least 1 year at −20°C. The tetra-n-butylammonium salts of nucleoside mono-, di-, or triphosphates are reacted with the SnISs to form reactive phosphoryl imidazolium donors. These donors are reacted with the tetra-n-butylammonium salts of pyrophosphate, nucleotide mono- or diphosphates or sugar-1-phosphates to give the nucleoside polyphosphates and their conjugates in excellent yields. Curr. Protoc. Nucleic Acid Chem. 51:13.11.1-13.11.24. © 2012 by John Wiley & Sons, Inc.
- nucleoside triphosphates;
- dinucleoside polyphosphates;
- sugar nucleotides;
- sulfonylimidazolium salts