UNIT 13.12 A Simple Chemical Synthesis of Sugar Nucleoside Diphosphates in Water
Published Online: 8 OCT 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Tanaka, H., Yoshimura, Y. and Hindsgaul, O. 2013. A Simple Chemical Synthesis of Sugar Nucleoside Diphosphates in Water. Current Protocols in Nucleic Acid Chemistry. 54:13.12:13.12.1–13.12.10.
- Published Online: 8 OCT 2013
Chemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection-deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio- and stereospecificity, but also the glycosyl donor to provide the sugar that is added. This unit describes a simple synthesis of sugar-nucleoside diphosphates (sugar-NDPs), the type of glycosyl donor (e.g., UDP-Glc, UDP-Gal, ADP-Glc) required by most glycosyltransferases, by using a chemical coupling reaction in water. The preparation of sugar-NDPs by this method therefore does not require any skills in synthetic organic chemistry. Curr. Protoc. Nucleic Acid Chem. 54:13.12.1-13.12.10. © 2013 by John Wiley & Sons, Inc.
- chemical synthesis;
- chemoenzymatic oligosaccharide synthesis;
- pyrophosphate formation;
- sugar-nucleoside diphosphate;
- sugar nucleotide