UNIT 13.13 Chemical Synthesis of Dinucleotide Cap Analogs
Published Online: 19 DEC 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Kore, A. R. and Shanmugasundaram, M. 2013. Chemical Synthesis of Dinucleotide Cap Analogs. Current Protocols in Nucleic Acid Chemistry. 55:13.13:13.13.1–13.13.12.
- Published Online: 19 DEC 2013
This unit describes a reliable, efficient and general method for the synthesis of standard cap analog (mCAP), m7G[5′]ppp[5′]G, and anti–reverse cap analog (ARCA), m7,3′OG[5′]ppp[5′]G. The synthesis of required intermediate m7GDP or m27,3′OGDP has been achieved through regioselective methylation of the corresponding diphosphate using dimethyl sulfate under aqueous conditions. Then, the coupling reaction of m7GDP or m27,3′OGDP with ImGMP using ZnCl2/DMF system affords the corresponding cap analog in good yields. Curr. Protoc. Nucleic Acid Chem. 55:13.13.1-13.13.12. © 2013 by John Wiley & Sons, Inc.
- cap analog;
- anti–reverse cap analog;
- regioselective methylation;
- eukaryotic mRNA;
- mRNA translation