Unit

UNIT 14.8 One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP)

  1. Barbara L. Gaffney,
  2. Roger A. Jones

Published Online: 1 MAR 2012

DOI: 10.1002/0471142700.nc1408s48

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Gaffney, B. L. and Jones, R. A. 2012. One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP). Current Protocols in Nucleic Acid Chemistry. 48:14.8:14.8.1–14.8.7.

Author Information

  1. Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey

Publication History

  1. Published Online: 1 MAR 2012
  2. Published Print: MAR 2012

Abstract

The bacterial signaling molecule, cyclic diguanosine monophosphate (c-di-GMP), plays a key role in controlling biofilm formation and pathogenic virulence, among many other functions. It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one-flask, gram-scale synthesis of c-di-GMP described here will facilitate these efforts. Curr. Protoc. Nucleic Acid Chem. 48:14.8.1-14.8.7. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • c-di-GMP;
  • bacterial signaling;
  • chemical synthesis