Miscellaneous
UNIT 15.1 Synthesis of Amino Acid Phosphoramidate Monoesters via H-Phosphonate Intermediates
Published Online: 1 JUL 2006
DOI: 10.1002/0471142700.nc1501s25
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
Additional Information
How to Cite
Choy, C. J., Drontle, D. P. and Wagner, C. R. 2006. Synthesis of Amino Acid Phosphoramidate Monoesters via H-Phosphonate Intermediates. Current Protocols in Nucleic Acid Chemistry. 25:15.1.1–15.1.12.
Publication History
- Published Online: 1 JUL 2006
- Published Print: JUN 2006
- Abstract
- Article
- Figures
- References
Diphenyl phosphite and bis(N,N-diisopropylamino)chlorophosphine are used as phosphitylating reagents to generate H-phosphonate monoesters. These H-phosphonate intermediates are subsequently oxidized with iodine to generate the 5¢-nucleoside amino acid phosphoramidates.
Keywords: diphenyl phosphite; bis(N,N-diisopropylamino)chlorophosphine; phosphoramidate; AZT; nucleoside monophosphate; oxidation