UNIT 16.1 Overview of Gene Silencing by RNA Interference
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Kim, D. H. and Rossi, J. J. 2009. Overview of Gene Silencing by RNA Interference. Current Protocols in Nucleic Acid Chemistry. 36:16.1:16.1.1–16.1.10.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
The potential of harnessing RNA interference (RNAi) for sequence-specific gene silencing has generated much excitement and progress in the field. Recent advances in RNAi technology suggest that RNAi-based approaches may soon become an effective therapeutic strategy against a myriad of diseases. This overview provides a brief description of important considerations when designing an RNAi-based method for gene silencing and therapeutic development: (a) mechanistic aspects of RNAi-mediated gene silencing in mammalian cells; (b) structural requirements for potent siRNA duplexes; (c) off-target effects and interferon responses; and (d) effective delivery of RNAi-inducing agents. Promising therapeutic applications of RNAi that are currently in the developmental pipeline are also described. Curr. Protoc. Nucleic Acid Chem. 36:16.1.1-16.1.10. © 2009 by John Wiley & Sons, Inc.
- transcriptional gene silencing