Unit

UNIT 17.1 UV Melting of G-Quadruplexes

  1. Jean-Louis Mergny,
  2. Laurent Lacroix

Published Online: 1 JUN 2009

DOI: 10.1002/0471142700.nc1701s37

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Mergny, J.-L. and Lacroix, L. 2009. UV Melting of G-Quadruplexes. Current Protocols in Nucleic Acid Chemistry. 37:17.1:17.1.1–17.1.15.

Author Information

  1. Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France

Publication History

  1. Published Online: 1 JUN 2009
  2. Published Print: JUN 2009

Abstract

Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (Tm values) and model-dependent thermodynamic parameters. Curr. Protoc. Nucleic Acid Chem. 37:17.1.1-17.1.15. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • quartets;
  • thermodynamics;
  • van't Hoff analysis;
  • thermal melting