Appendix

APPENDIX 3C Introduction to the Synthesis and Purification of Oligonucleotides

  1. Andrew Ellington1,
  2. Jack D. Pollard Jr.2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nca03cs00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Ellington, A. and Pollard, J. D. 2001. Introduction to the Synthesis and Purification of Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 00:3C:A.3C.1–A.3C.22.

Author Information

  1. 1

    University of Texas, Austin, Texas

  2. 2

    Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo- and oligoribonucleotides with a variety of labels, modified linkages, and nonstandard bases. Strategies are given for the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection.