APPENDIX 3D Thin-Layer Chromatography

  1. C.L.F. Meyers,
  2. D.J. Meyers

Published Online: 1 SEP 2008

DOI: 10.1002/0471142700.nca03ds34

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Meyers, C. and Meyers, D. 2008. Thin-Layer Chromatography. Current Protocols in Nucleic Acid Chemistry. 34:3D:A.3D.1–A.3D.13.

Author Information

  1. Johns Hopkins University School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 SEP 2008
  2. Published Print: SEP 2008


TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values. Curr. Protoc. Nucleic Acid Chem. 34:A.3D.1-A.3D.13. © 2008 by John Wiley & Sons, Inc.


  • thin-layer chromatography;
  • TLC;
  • nucleosides;
  • nucleotides;
  • separation