APPENDIX 3D Thin-Layer Chromatography
Published Online: 1 SEP 2008
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Meyers, C. and Meyers, D. 2008. Thin-Layer Chromatography. Current Protocols in Nucleic Acid Chemistry. 34:3D:A.3D.1–A.3D.13.
- Published Online: 1 SEP 2008
- Published Print: SEP 2008
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values. Curr. Protoc. Nucleic Acid Chem. 34:A.3D.1-A.3D.13. © 2008 by John Wiley & Sons, Inc.
- thin-layer chromatography;