APPENDIX 3E Column Chromatography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Meyers, C. 2001. Column Chromatography. Current Protocols in Nucleic Acid Chemistry. 3:3E:A.3E.1–A.3E.7.
- Published Online: 1 MAY 2001
- Published Print: JAN 2001
Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described include loading the column, assembling the apparatus, separating compounds and collecting fractions, and optimizing chromatography conditions.