Appendix

APPENDIX 3E Column Chromatography

  1. C.L.F. Meyers

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nca03es03

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Meyers, C. 2001. Column Chromatography. Current Protocols in Nucleic Acid Chemistry. 3:3E:A.3E.1–A.3E.7.

Author Information

  1. Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described include loading the column, assembling the apparatus, separating compounds and collecting fractions, and optimizing chromatography conditions.