APPENDIX 3G Techniques for Mammalian Cell Tissue Culture
Published Online: 1 NOV 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Phelan, M. C. 2006. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Human Genetics. 51:3G:A.3G.1–A.3G.18.
- Published Online: 1 NOV 2006
- Published Print: OCT 2006
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
- mammalian cells;
- cell culture;