Unit

UNIT 1.2 Growth in Liquid Media

  1. Karen Elbing1,
  2. Roger Brent2

Published Online: 1 AUG 2002

DOI: 10.1002/0471142727.mb0102s59

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Elbing, K. and Brent, R. 2002. Growth in Liquid Media. Current Protocols in Molecular Biology. 59:I:1.2:1.2.1–1.2.2.

Author Information

  1. 1

    Clark & Elbing LLP, Boston, Massachusetts

  2. 2

    The Molecular Sciences Institute, Berkeley, California

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUL 2002

Abstract

The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.