UNIT 1.6 Minipreps of Plasmid DNA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Engebrecht, J., Brent, R. and Kaderbhai, M. A. 2001. Minipreps of Plasmid DNA. Current Protocols in Molecular Biology. 15:II:1.6:1.6.1–1.6.10.
- Published Online: 1 MAY 2001
- Published Print: JUL 1991
Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.