Unit

UNIT 1.6 Minipreps of Plasmid DNA

  1. JoAnne Engebrecht1,
  2. Roger Brent2,
  3. Mustak A. Kaderbhai (lithium miniprep)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0106s15

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Engebrecht, J., Brent, R. and Kaderbhai, M. A. 2001. Minipreps of Plasmid DNA. Current Protocols in Molecular Biology. 15:II:1.6:1.6.1–1.6.10.

Author Information

  1. 1

    State University of New York, Stony Brook, New York

  2. 2

    The Molecular Sciences Institutes, Berkeley, California

  3. 3

    University College of Wales, Penglais, Aberystwyth, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1991

Abstract

Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.