UNIT 1.8 Introduction of Plasmid DNA into Cells

  1. Christine E. Seidman (calcium)1,
  2. Kevin Struhl (one-step)1,
  3. Jen Sheen (electroporation)2,
  4. Timm Jessen (direct transfer)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0108s37

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Seidman, C. E., Struhl, K., Sheen, J. and Jessen, T. 2001. Introduction of Plasmid DNA into Cells. Current Protocols in Molecular Biology. 37:II:1.8:1.8.1–1.8.10.

Author Information

  1. 1

    Havard Medical School, Boston, Massachusetts

  2. 2

    Massachusetts General Hospital, Boston, Massachusetts

  3. 3

    Hoechst AG, Frankfurt am Main, Germany

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1997


Transformation of E. coli can be achieved using any of the four protocols in this unit. The first method using calcium chloride gives good transformation efficiencies, is simple to complete, requires no special equipment, and allows storage of competent cells. The alternate one-step method is considerably faster and also gives good transformation efficiencies (although they are somewhat lower). If considerably higher transformation efficiencies are needed, the third method using electroporation is simple, fast, and reliable. As in the calcium chloride protocol, prepared cells can be stored. The final method described is an adaptation of the electroporation protocol that allows direct transfer of vector DNA from yeast into E. coli.