UNIT 1.8 Introduction of Plasmid DNA into Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Seidman, C. E., Struhl, K., Sheen, J. and Jessen, T. 2001. Introduction of Plasmid DNA into Cells. Current Protocols in Molecular Biology. 37:II:1.8:1.8.1–1.8.10.
- Published Online: 1 MAY 2001
- Published Print: JAN 1997
Transformation of E. coli can be achieved using any of the four protocols in this unit. The first method using calcium chloride gives good transformation efficiencies, is simple to complete, requires no special equipment, and allows storage of competent cells. The alternate one-step method is considerably faster and also gives good transformation efficiencies (although they are somewhat lower). If considerably higher transformation efficiencies are needed, the third method using electroporation is simple, fast, and reliable. As in the calcium chloride protocol, prepared cells can be stored. The final method described is an adaptation of the electroporation protocol that allows direct transfer of vector DNA from yeast into E. coli.