Unit

UNIT 1.10 Lambda as a Cloning Vector

  1. Karen Lech1,
  2. Roger Brent1,
  3. Nina Irwin2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0110s00

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lech, K., Brent, R. and Irwin, N. 2001. Lambda as a Cloning Vector. Current Protocols in Molecular Biology. 00:III:1.10:1.10.1–1.10.11.

Author Information

  1. 1

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    Harvard University, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1987

Abstract

Advantages of lambda as a cloning vector are discussed along with considerations for the insert DNA (i.e., size, spi and hfl state). Maps of lambda-derived cloning vectors are provided in addition to a discussion and map of a cosmid (a lambda-derived plasmid vector).