Unit

UNIT 1.11 Plating Lambda Phage to Generate Plaques

  1. Karen Lech,
  2. Roger Brent

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0111s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lech, K. and Brent, R. 2001. Plating Lambda Phage to Generate Plaques. Current Protocols in Molecular Biology. 00:III:1.11:1.11.1–1.11.4.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991

Abstract

This unit provides detailed protocols for isolating a single plaque by titering serial dilutions or streaking on a lawn of cells. A discussion of phage transfection and in vitro packaging is also included.