Unit

UNIT 1.12 Growing Lambda-Derived Vectors

  1. Karen Lech

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0112s13

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lech, K. 2001. Growing Lambda-Derived Vectors. Current Protocols in Molecular Biology. 00:III:1.12:1.12.1–1.12.3.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1991

Abstract

It is often necessary to grow large quantities of lambda-derived vectors, so that DNA can be made from them. This unit describes a procedure for making a phage stock by plate lysis, and an Alternate Protocol gives details on how to make a liquid lysate. Storage of phage lysates is described in a Support Protocol.