Unit

UNIT 1.13 Preparing Lambda DNA from Phage Lysates

  1. Karen Lech1,
  2. K.J. Reddy2,
  3. L.A. Sherman (column purification)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0113s10

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Lech, K., Reddy, K. and Sherman, L. 2001. Preparing Lambda DNA from Phage Lysates. Current Protocols in Molecular Biology. 10:III:1.13:1.13.1–1.13.10.

Author Information

  1. 1

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1990

Abstract

DNA extracted from lambda-derived vectors is typically subcloned into plasmid or filamentous phage vectors. The first two protocols describe methods for isolating phage DNA from large- and medium-scale liquid lysates. These two methods use either density-gradient centrifugation or ion-exchange chromatography to purify the phage particles. The third protocol describes a rapid procedure for isolating phage DNA, suitable for small-scale liquid lysates.