UNIT 1.13 Preparing Lambda DNA from Phage Lysates
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Lech, K., Reddy, K. and Sherman, L. 2001. Preparing Lambda DNA from Phage Lysates. Current Protocols in Molecular Biology. 10:III:1.13:1.13.1–1.13.10.
- Published Online: 1 MAY 2001
- Published Print: APR 1990
DNA extracted from lambda-derived vectors is typically subcloned into plasmid or filamentous phage vectors. The first two protocols describe methods for isolating phage DNA from large- and medium-scale liquid lysates. These two methods use either density-gradient centrifugation or ion-exchange chromatography to purify the phage particles. The third protocol describes a rapid procedure for isolating phage DNA, suitable for small-scale liquid lysates.