UNIT 1.15 Preparing and Using M13-Derived Vectors

  1. David Greenstein1,
  2. Claude Besmond2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0115s09

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Greenstein, D. and Besmond, C. 2001. Preparing and Using M13-Derived Vectors. Current Protocols in Molecular Biology. 00:IV:1.15:1.15.1–1.15.8.

Author Information

  1. 1

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  2. 2

    Centre de Recherche Inserm, Paris, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1990


Many vectors in current use are derived from filamentous phages. These vectors are used because DNA inserted into them can be recovered in two forms: double-stranded circles and single-stranded circles. This overview unit describes the lifecycle of filamentous phages along with the development and use of filamentous phage vectors.