Unit

UNIT 1.17  E. coli Genome Manipulation by P1 Transduction

  1. Lynn C. Thomason,
  2. Nina Costantino,
  3. Donald L. Court

Published Online: 1 JUL 2007

DOI: 10.1002/0471142727.mb0117s79

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Thomason, L. C., Costantino, N. and Court, D. L. 2007. E. coli Genome Manipulation by P1 Transduction. Current Protocols in Molecular Biology. 79:V:1.17:1.17.1–1.17.8.

Author Information

  1. National Cancer Institute at Frederick, Frederick, Maryland

Publication History

  1. Published Online: 1 JUL 2007
  2. Published Print: JUL 2007

Abstract

This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another. Fragments of ∼100 kb can be transferred by the P1 bacteriophage. The phage is first grown on a strain containing the elements to be moved, and the resulting phage lysate is used to infect a second recipient strain. The lysate will contain bacterial DNA as well as phage DNA, and genetic recombination, catalyzed by enzymes of the recipient strain, will incorporate the bacterial fragments into the recipient chromosome. Curr. Protoc. Mol. Biol. 79:1.17.1-1.17.8. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • transduction;
  • E. coli bacteriophage P1;
  • genetic recombination