UNIT 1.17 E. coli Genome Manipulation by P1 Transduction
Published Online: 1 JUL 2007
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Thomason, L. C., Costantino, N. and Court, D. L. 2007. E. coli Genome Manipulation by P1 Transduction. Current Protocols in Molecular Biology. 79:V:1.17:1.17.1–1.17.8.
- Published Online: 1 JUL 2007
- Published Print: JUL 2007
This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another. Fragments of ∼100 kb can be transferred by the P1 bacteriophage. The phage is first grown on a strain containing the elements to be moved, and the resulting phage lysate is used to infect a second recipient strain. The lysate will contain bacterial DNA as well as phage DNA, and genetic recombination, catalyzed by enzymes of the recipient strain, will incorporate the bacterial fragments into the recipient chromosome. Curr. Protoc. Mol. Biol. 79:1.17.1-1.17.8. © 2007 by John Wiley & Sons, Inc.
- E. coli bacteriophage P1;
- genetic recombination