Unit

UNIT 2.1B Purification of DNA by Anion-Exchange Chromatography

  1. Kim Budelier1,
  2. Joachim Schorr2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0201bs42

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Budelier, K. and Schorr, J. 2001. Purification of DNA by Anion-Exchange Chromatography. Current Protocols in Molecular Biology. 42:I:2.1B:2.1.11–2.1.18.

Author Information

  1. 1

    QIAGEN, Inc., Valencia, California

  2. 2

    QIAGEN GmbH, Hilden, Germany

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1998

Abstract

Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The procedure employs columns supplied by QIAGEN; other preparation methods are available from other suppliers. A crude nucleic acid sample (usually a cleared cell lysate) is applied to the QIAGEN tip under conditions that favor binding. Contaminants in the sample are washed from the column with a moderate-salt buffer, and DNA is eluted using a high-salt buffer.