Unit

UNIT 2.2 Preparation of Genomic DNA from Mammalian Tissue

  1. William M. Strauss

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0202s42

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Strauss, W. M. 2001. Preparation of Genomic DNA from Mammalian Tissue. Current Protocols in Molecular Biology. 42:I:2.2:2.2.1–2.2.3.

Author Information

  1. Harvard Medical School and Beth, Israel Deaconess Medical Center, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1998

Abstract

There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteination and recovery of DNA. The main differences between various approaches lie in the extent of deproteination and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.