Unit

UNIT 2.3 Preparation of Genomic DNA from Plant Tissue

  1. Eric Richards1,
  2. Mark Reichardt2,
  3. Sharon Rogers2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0203s27

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Richards, E., Reichardt, M. and Rogers, S. 2001. Preparation of Genomic DNA from Plant Tissue. Current Protocols in Molecular Biology. 27:I:2.3:2.3.1–2.3.7.

Author Information

  1. 1

    (CsCl preparation) Washington University, St. Louis, Missouri

  2. 2

    (CTAB preparation) Lakeside Biotechnology, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1994

Abstract

This unit describes two methods for preparing genomic DNA from plant tissue. In the first method, plant cells are lysed with ionic detergent, treated with protease, and subsequently purified by cesium chloride (CsCl) density gradient centrifugation. The second method is based upon a series of treatments with the nonionic detergent cetyltrimethylammonium bromide (CTAB) to lyse cells and purify nucleic acid. Nucleic acid is recovered from the final CTAB solution by isopropanol or ethanol precipitation. The first method, although somewhat more lengthy, results in highly purified nucleic acid. The second method requires fewer manipulations, results in very high yields (˜10-fold higher per gram fresh tissue depending on species and condition of starting material), and produces DNA that is less pure but nonetheless suitable in quality for use in many molecular biology manipulations.