Unit

UNIT 2.5A Agarose Gel Electrophoresis

  1. Daniel Voytas

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0205as51

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Voytas, D. 2001. Agarose Gel Electrophoresis. Current Protocols in Molecular Biology. 51:II:2.5A:2.5A.1–2.5A.9.

Author Information

  1. Iowa State University, Ames, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5- to 25-kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample wells and the gel is run at a voltage and for a time period that will achieve optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis buffer, visualized directly upon illumination with UV light.