UNIT 2.8 Capillary Electrophoresis of DNA
Published Online: 1 NOV 2004
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Smith, A. and Nelson, R. J. 2004. Capillary Electrophoresis of DNA. Current Protocols in Molecular Biology. 68:III:2.8:2.8.1–2.8.17.
- Published Online: 1 NOV 2004
- Published Print: OCT 2004
Capillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, sensitivity, and data handling. Separation times are generally only a few minutes and the DNA is detected either by UV absorption or by fluorescent labeling. The quantity of DNA required for separation is in the nanogram range. Single-base resolution can be obtained on fragments up to several hundred base pairs. In the presence of appropriate standards, fragments can be accurately sized based on relative electrophoretic mobility. A protocol for the analysis of synthetic oligonucleotides in a flowable matrix is described in this unit.