UNIT 2.11 Synthesis and Purification of Oligonucleotides

  1. Andrew Ellington1,
  2. Jack D. Pollard Jr.2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0211s42

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Ellington, A. and Pollard, J. D. 2001. Synthesis and Purification of Oligonucleotides. Current Protocols in Molecular Biology. 42:V:2.11:2.11.1–2.11.25.

Author Information

  1. 1

    University of Texas, Austin, Texas

  2. 2

    Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1998


This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.