Miscellaneous
UNIT 2.11 Synthesis and Purification of Oligonucleotides
Published Online: 1 MAY 2001
DOI: 10.1002/0471142727.mb0211s42
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
Additional Information
How to Cite
Ellington, A. and Pollard, J. D. 2001. Synthesis and Purification of Oligonucleotides. Current Protocols in Molecular Biology. 42:2.11.1–2.11.25.
Publication History
- Published Online: 1 MAY 2001
- Published Print: APR 1998
- Abstract
- Article
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This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.