Unit

UNIT 2.12 Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis

  1. Andrew Ellington1,
  2. Jack D. Pollard Jr.2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142727.mb0212s42

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Ellington, A. and Pollard, J. D. 2001. Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis. Current Protocols in Molecular Biology. 42:V:2.12:2.12.1–2.12.7.

Author Information

  1. 1

    University of Texas, Austin, Texas

  2. 2

    Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1998

Abstract

Cloning vectors derived from filamentous phage are extremely useful because they allow cloned DNA to be isolated as either single- or double-stranded DNA. This unit contains protocols for preparing both forms of DNA and for characterizing inserts in M13-derived vectors. A protocol is also presented for preparing single-stranded DNA from plasmids using superinfection with helper phage. This method is advantageous because it allows cloned DNA to be maintained in the form of a plasmid while permitting single-stranded DNA to be isolated for DNA sequencing.