Unit

UNIT 2.14 DNA Isolation from Mammalian Samples

  1. Ximeng Liu,
  2. Shuko Harada

Published Online: 1 APR 2013

DOI: 10.1002/0471142727.mb0214s102

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Liu, X. and Harada, S. 2013. DNA Isolation from Mammalian Samples. Current Protocols in Molecular Biology. 102:2.14.1–2.14.13.

Author Information

  1. Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples. Curr. Protoc. Mol. Biol. 102:2.14.1–2.14.13. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • DNA isolation;
  • silica spin column;
  • FFPE (formalin-fixed paraffin-embedded) tissue;
  • automated extraction