UNIT 2.14 DNA Isolation from Mammalian Samples
Published Online: 1 APR 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
Liu, X. and Harada, S. 2013. DNA Isolation from Mammalian Samples. Current Protocols in Molecular Biology. 102:VI:2.14:2.14.1–2.14.13.
- Published Online: 1 APR 2013
- Published Print: APR 2013
DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples. Curr. Protoc. Mol. Biol. 102:2.14.1–2.14.13. © 2013 by John Wiley & Sons, Inc.
- DNA isolation;
- silica spin column;
- FFPE (formalin-fixed paraffin-embedded) tissue;
- automated extraction